Advanced Electron Microscopy Facility

Advanced Electron Microscopy Core Facility
Gordon Center for Integrated Science
929 E. 57th St.
Rooms: ESB07, ESB23 (Scope room)
Chicago, IL 60637

Technical contact:
Jotham (Joe) Austin
773-702-9091

A primary mission of the Advanced Electron Microscopy Facility is to develop techniques for preserving cellular structure with the highest degree of reliability. These techniques involve different methods for rapidly freezing our samples in order to halt structural and biochemical activity in a very short timeframe, thus preserving structure in the "live" state. Once the sample is preserved in the "live" state, it is then possible to study the ultrastructure of these samples using not only basic Electron Microscopy imaging techniques, but also state-of the-art techniques such as: 1) 3-D electron tomography, and 2) immuno-cytochemistry.Baltec HPM01 High Pressure Freezing Machine

FEI Tecnai G2 F30 Super Twin microscope

 

The FEI Tecnai F30 is a scanning transmission electron microscope (STEM) that forms the centerpiece of the Electron Microscopy Core Facility. The microscope can be operated at the maximum accelerating voltage of 300 kV and is capable of atomic resolution imaging in both TEM and STEM modes. The microscope uses a Schottky field emission gun as its electron source, providing the highest current density and the smallest electron probe-size, which are critical for high spatial-resolution analysis using the scanning mode.The microscope is currently equipped with a high performance CCD camera with 4k x 4k resolution for image recording, an X-ray energy dispersive spectrometer (EDX) for compositional analysis, and STEM detectors for bright-field, annular dark-field, and high-angle annual dark-field (Z-contrast) imaging.

High brightness Schottky emitter operated at 300 kV
digital CCD camera with 4k x 4k image resolution
high-resolution TEM imaging with the point-to-point resolution of 0.2 nm
STEM imaging at the spatial resolution of 0.2 nm
EDX for elemental analysis
Standard single-tilt and low-background (Be) double-tilt holders
low dose capability
tomography capability

Fischione Dual-Axis Tomography Holder Model 2040

 

Leica EM AFS2 Automatic Freeze-Substitution Processor

 

Leica UC6 Ultramicrotome with MZ6 Microscope and Accessories

 

Sample Equipment

Ultracut E Microtome for sectioning
Edwards Auto 306 Evaporator for carbon coating and glow discharge

Software

Gatan DigitalMicrograph
Focal-Series High-Resolution Reconstruction Package
Tomography Acquisition and Reconstruction Package

https://tomocryo.uchicago.edu/